Use of Caenorhabditis elegans, Arthrobotrys oligospora and Bacillus subtilis interaction in control of Meloidogyne javanica

Caenorhabditis elegans، Arthrobotrys oligospora and Bacillus subtilis interaction were studied to control root knot nematode، Meloidogyne javanica. B. subtilis used for inducing of plant defense system at the initiation of treatment and as the C. elegans consumption. C. elegans also used in order to induce the production of traps. A. oligospora caused 77% larval mortality، after 72 hours. Extracellular protease production was evaluated 2 units in compared to control. All treatments significantly reduced the number of knots and egg masses per plant in compared to control، but the minimum level of these indexes observed in treatment of B. subtilis+C. elegans+A. oligospora against M. javanica. The effects of volatile، non volatile compounds and growth inhibition percentage of B. subtilis against A. oligospora showed that non volatile compounds (antibiotics) were more effective than other tested inhibition mechanisms.