Isolation and cloning of human Developmental pluripotency associated-2 cancer testis gene

Cancer/testis antigens (CTAs) are a subgroup of tumor-associated antigens which are expressed in trophoblast، ovary and testis germ line cells. CTAs are also expressed in a variety of cancer cells and their biological function in germ line and tumors cells is unclear yet. One of the most important CTAs is CT100 which is also known as DPPA2. The aim of this study was isolation، amplification and cloning of DPPA2 coding sequence. Having examined the gene expression pattern of different cell lines، we amplified coding sequence of DPPA2 gene and cloned it in pTZ57R/T vector. Recombinant colonies were analyzed and confirmed by colony PCR، double digestion and sequencing. Considering the importance of DPPA2 roles in the development، its role analysis in carcinogenesis seems valuable. Our results prepare a suitable platform to analyze its biological function.