The Effect of high intensity intermittent trainings on brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor levels in brain of rats

Researches have showed that exercise trainings increase the brain-derived neurotrophic (BDNF) and glial cell-derived neurotrophic factor (GDNF) in the brain. In addition, hydrogen peroxide (H2O2) and tumor necrosis factor alpha (TNFα) increase protein content of BDNF and GDNF in vitro. However, there is insufficient information about the interactive effects of high intensity exercise training, H2O2, and TNFα on neurotrophins. Hence, in the present study, we investigated the effect of high intensity intermittent training on the content of BDNF, GDNF, H2O2 and TNFα in the brain of albino wistar rats. Sixteen albino wistar rats divided into control and high intensity intermittent training groups. High intensity intermittent training has carried out for 6 weeks with 95 to 100% of maximum oxygen consumption on treadmill. BDNF, GDNF and TNFα contents have measured by sandwich ELISA method and H2O2 concentration by colorimetric method by commercial kits. Data analyzed using Student’s t-test, and p≤0.05 considered as statistically significant. High intensity intermittent training resulted in 75 and 143 percent increased in H2O2 (p<0.0001) and TNFα (p<0.0001) levels, which accordance with 149 and 170 percent increase in BDNF (p<0.0001) and GDNF (p<0.0001) content in the brain, respectively. Overall, performance of training sessions as interval with maximum capacity resulting in increase of BDNF and GDNF content in brain of albino wistar rat and it seems H2O2 and TNFα influence on neurotrophins adaptation induced by high intensity intermittent training.