Detection of Transgenic Roundup Ready Soybean Seeds by Molecular Methods

As genetically modified organisms (GMOs) development is now increasing, detection and determination of their quantitative threshold using reliable and sensitive methods would be necessary. The aim of this study was to introduce reliable methods for qualitative and quantitative detection of Roundup-tolerant soybean particularly at event level via molecular approaches. For primary screening, we used polymerase chain reaction with several element–specific primers for detection of different percent of transgenic and non-transgenic Roundup ready soybean seed samples. Furthermore, semi-quantitative assays were performed using the same primers in combinations with soybean lectin-specific primers. The limit of detection (LOD) obtained for CTP-35S construct and event-specific (pDNA-35S) was 5%. This index for cp4 epsps and nos terminator sequences was 0.5% and for CaMV 35S promoter was 1%. Results showed that the sensitivity of analysis for nos terminator and cp4 epsps sequences was more than other elements and therefore, this method could be applied for rapid screening of Roundup Ready transgenic soybean samples.