Study of In vitro Shoot Proliferation and Rhizogenesis of Almond and Peach Hybrid (GN15) Rootstock

GN15 (Garnem) is a vigorous rootstock, used for peach, nectarine and almond and is suitable for many areas of Iran. In this study, effect of 6-benzyladenine (BA) at 2, 4 and mg l-1 in combination with 1-naphthaleneacetic acid (NAA) at 0, 0.05 and 0.1 mg l-1 in WPM medium on shoot proliferation of GN15 rootstock were studied. The results showed the highest number of proliferated shoot per explant in 2 mg l-1 BA and 0.1 mg l-1 NAA. Maximum length of shoots was observed in treatment with 4 mg l-1 BA without NAA and number of nodes was increased in 2 mg l-1 BA and 0 mg l-1 NAA. Maximum length of internodes was obtained in medium containing 2 mg l-1 BA and 0.1 mg l-1 NAA. Increasing concentration of BA up to 8 mg l-1 without NAA increased vitrification and decreased shoot number per explants, shoot length, nodes number and internode length. Kinetin treatments had limited effect on proliferation. The influence of two basal medium, MS and 1/2MS with different concentrations of IBA and NAA were investigated on rhizogenesis. MS medium supplemented with 1 mg l-1 IBA was most effective for rooting percentage. Maximum number and length of roots per explants were seen in 1/2 MS medium supplemented with 1 mg l-1 NAA and 2 mg l-1 IBA, respectively.