Survey the Existence of CagA in Helicobacter Pylori Levels Separated from Patients Suffering from the Lower Gastrointestinal Disorders by PCR Method

Helicobacter pylori is the main reason of different gastrointestinal diseases. Studies have shown that the diversity of infection outcomes resulted from H. pylori may be associated with differences in genotypes or bacterial pathogenesis factors expression and also environmental and host factors in isolated H. pylori strains in patients with CagA and VacA. Thepurpose of this study is to determine the frequency of cagA gene in helicobacter pylori levels separated from patients suffering from lower gastrointestinal disorders. This study is a cross- sectional descriptive one, carried out on 127 patients referred to gasterointestinal clinic of Guilan Province, Gastrointestinal and Liver Research Center, in which the frequency of cagA gene in 50 separated helicobacter pylori cases was investigated using polymeras chain reaction (PCR). The used statistical test was Chai Square. Among the studied samples, in 50 cases bacteria were isolated by culture method. After performing PCR, the frequencies of cagA gene in strains from patients with mild antral gastritis, erosive gastritis, duodenal ulcer, gastric ulcer were equivalent to 85.7% 64.3%, 100% and 100%, respectively. There were significant differences in the association of this gene with isolated strains from patients with erosive gastritis (p<0.05), but the difference was not significant for the isolated strains from patients with mild antral gastritis, duodenal ulcer, and gastric ulcer. This study has shown that H. pylori cagA gene level was higher among patients with gastric ulcer and duodenal ulcers.