Introduction of Pellet Culture System of Human Adipose- Derived Mesenchymal Stem Cells as an In Vitro Model for Cartilage Engineering Approaches

Various diseases and injuries can lead to loss cartilage tissue. Cartilage tissue engineering based on the use of stem cells which has provided a promising opportunity to repair damaged tissue. Recently، adipose-derived mesenchymal stem cells (ADSCs) have captured considerable scientific and clinic interest because of their easy access، rapid expansion in vitro and ability to differentiate into diverse cell lines. The aim of this study was to introduce the pellet culture system as an in vitro model for cartilage engineering approaches. This experimental study was performed in Stem Cell laboratory، The Academic Center for Education، Culture and Research، Qom in 2013. In this study، ADSCs were isolated and expanded from human adipose tissue. The cells were centrifuged and the chondrogenic differentiation was performed in pellet culture system. Viability potential and chondrogenic genes expression were evaluated by MTT assay and Real-time PCR analysis، respectively. In this study، data statistical analysis was carried out by independent T-test. In this study، our obtained results using MTT assay and Real-time PCR analysis demonstrated cell survival ability and proliferation and also the expression of chondrogenic-specific genes at pellet in comparison with control group. It is suggested that this method can be used for induction of chondrogenic.