Prevalence of Virulence Genes etA, etB in Community Acquired Methicillin-Resistant Staphylococcus aureus in Patients Referred to Teaching Hospital by PCR in Shahrekord, 2014

Colonization with MRSA is no longer limited to hospitalized patients or persons with predisposing risk factors and at present there are several strains of community-acquired MRSA (CA-MRSA). The purpose of this study is to determine etA and etB genes in isolated Staphylococcus aureus strains of clinical samples from teaching hospital in Shahrekord in 2014. In this descriptive study, 220 clinical samples were collected from teaching hospitals in Shahrekord. The microbiologic characteristics of isolates were determined using microbiologyic standard methods. The MRSA detection was carried out on oxacillin agar medium. The detection of virulence genes etA and etB was used by PCR. Inducible resistance to clindamycin was tested by "D-test". In 220 isolates, 110 detected as S. aureus and 13 as MRSA. Inducible clindamycin resistance was seen in 4 (3.5%) of the isolates. The frequency of genes etA, etB in studied strains was 7.6 and 15.3, respectively. Also, Inducible resistance to clindamycin was seen in four isolates(%2). The results of this study confirme the presence of community- acquired strains in Shahrekord. The results of this study indicate the presence of genes etA and etB in the strains studied, transforming into the strains of Staphylococcus aureus resistant to methicillin obtained from hospital, the development and transfering these strains in the community.