EFFECTIVITY OF FERMENTED GOAT MILK ADDED LACTOBACILLUS PLANTARUM AS MELANIN INHIBITOR

This study aimed to investigate the affectivity of goat milk fermented with Lactobacillus plantarum (TW 14) as melanin inhibitor. Prior to the treatment of melanin inhibitor, samples were pasteurized. After that, the samples were incubated at 37 oC for 24 hour and analyzed for lactic acid bacteria population, total titratable acid, protein and fat contents. Fermented milk were extracted by centrifugation, the supernatant was collected and used for inhibition of tyrosinase enzymes activity and inhibitory melanin on B16F0 cell analysis. The results showed that the milk used complied with standard fermented milk which able to inhibit tyrosinase activity (L-DOPA Substrat). Goat milk was unable to inhibit melanin synthesis on B16F0 cell but fermented milk showed the ability, inhibit tyrosinase and melanin synthesis on B16F0 cell. A concentration fermented sample of 66.7 mg/ml resulted in a decrease in melanin concentration of extracellular in TW 14 treatment groups for 27%. In general goat milk fermented with lactic acid bacteria is an effective as melanin inhibitor.