Effects of Different Storage Temperatures and Times on Germination and Antioxidant Responses of Jatropha curcas Seeds
Abstract:
The effects of different storage temperatures and times on germination rate, MalonDiAldehyde (MDA), SuperOxide Dismutase (SOD), PerOxiDase (POD) and Catalase (CAT) activities in Jatropha curcas L. seed were investigated. The results showed that germination rates of seeds stored at room temperature and 4°C for 1 month was significantly higher than those stored for 3 months at -20 and -80°C. MDA contents increased significantly for 1 and 3 months storage with decreasing temperatures. SOD activity decreased gradually down to -80°C for 1 and 3 months storage. POD activity fluctuated slightly for 1 month, but increased apparently for 3 months storage with decreasing temperatures. CAT activity declined rapidly as storage temperatures decreased, particularly for 3 months storage. A significant interaction of storage temperatures and times was found for the activity of SOD and POD, and there was no significant interaction on germination rate, MDA content, and CAT activity. Electrophoretic analysis showed that the observed changes of SOD, POD and CAT isoenzyme bands under different storage temperatures were consistent with the changes of enzyme activities assayed in extract solutions. These results suggested that SOD, POD, and CAT may be involved in regulating the level of reactive oxygen species under different storage temperatures and times.
Keywords:
Language:
English
Published:
Journal of Agricultural Science and Technology, Volume:17 Issue: 6, Nov 2015
Pages:
1619 to 1628
magiran.com/p1460247
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!