The Synergic Effect of Chiton lamyi Foot Alcoholic Extract and Acellular Rat Brain's Tissue on Angiogenesis in Chick Embryo Chorioalantoic Membrane

In this study the synergic effect of Chiton lamyi foot alcoholic extract and decellulation rat brain’s tissue on angiogenesis in hen (chick?) chorioalantoic membrane (CAM) was investigated. In this study، 70 fertilized eggs of Ross strain were divided into 7 treatments groups in ten repetitions: 1. control، 2. DMSO، 3. decellulation rat brain’s tissue، 4. 10mgμl-1 Chiton lamyi foot alcoholic extract، 5. 20mgμl-1 Chiton lamyi foot alcoholic extract، 6. decellulation rat brain’s tissue & 10mgμl-1 Chiton lamyi foot alcoholic extract and 7. decellulation rat brain’s tissue & 20mgμl-1 Chiton lamyi foot alcoholic extract. All of eggs were placed in the incubator for eight days and treatments induction was done in 8th day. Then they photographed using photo steriomicroscope in 12th day. Blood vessels branches length and number were evaluated by Image J software in treatment sites on chorioalantoic membrane. Data were analyzed using ANOVA، SPSS Test (P<0. 05). Results showed that Chiton lamyi foot alcoholic extract، 10 and 20mgμl-1 Chiton lamyi foot alcoholic extracts with decellulation rat brain’s tissue treatments significantly decreased blood vessels branches length and number in comprising with control and DMSO treatments (P< 0. 05). The Synergic usage of Chiton lamyi foot alcoholic extract and decellulation (acellular) rat brain’s tissue has dose inhibitory effect on hen chorioalantoic membrane (CAM) angiogenesis.