Antagonistic effects of Fusarium spp. associated with females and egg masses on Meloidogyne javanica In in vitro conditions

In order to investigate the antagonistic effects of the fungi associated with adult females and egg masses of Meloidogyne javanica in tomato and cucumber grown in greenhouses of Kermanshah province, research experiments were performed in 2009-2010. Among isolated fungi there were 49 isolates of Fusarium spp. from which F. chlamydosporum, F. oxysporum, F. proliferatum, F. solani, F. tricinctum and F. verticillioides were identified. For purification and propagation of the nematode, single egg masses of adult females were inoculated on the roots of cucumber seedlings in the growth chamber. After nematode propagation, pathogenicity experiments were carried out in transparent plastic trays with a completely randomized design. Antagonistic effects of these fungal species were investigated on M. javanica through parameters including parasitized eggs percent, hatching eggs percent and juvenile(J2) mortality. F. chlamydosporum, F. solani and F. oxysporum had the highest ability to parasitizing the eggs. F. solani for its effect on preventing egg hatching and all Fusariums isolates for their effects on juvenile mortality had significant difference. In conclusion, F. solani had the highest antagonistic effects over others and also had the most abundance.