Evaluatiing the protective effect of Hypericum perforatum L. on some blood biochemical parameters in rates exposed to phenol

Because of the use of phenol in petrochemical industry and as a disinfectant effect in medicine, this material is widely used. However, the free phenol in the environment is detrimental to the health. The aim of present study was to evaluate the protective effect of Hypericum perforatum L. on some of the blood biochemical parameters in rats with toxicity induced by phenol. For this purpose, a number of 54 male Wistar rats were divided into 9 treatment groups as 3×3 factorial design with two main factors of Hypericum perforatum (at concentrations of 0, 250 and 500 mg/kg.bw) and phenol (at concentrations of 0, 100 and 200mg/kg.bw). Rats received the extract and phenol every other day with gavage method. On days 15 and 30 of experiment, after blood sampling, the concentrations of some blood serum biochemical parameters were evaluated. In first half of experiment, 200 mg/kg.bw of phenol significantly increased the blood serum cholesterol and LDL values and a concentration of 100mg/kg.bw reduced the VLDL level (p<0.05). In second half of experiment, the highest level of HDL was observed in treatment 250 and 200 mg/kg.bw Hypericum perforatum and phenol, respectively and its lowest value was recorded at concentrations of 500 and 200 mg/kg.bw Hypericum perforatum and phenol, respectively (p<0.05). The other main and interaction effects of treatments were not significant (p>0.05). The findings of present research show that the extract at a concentration of 250 mg/kg could decrease the adverse effect of phenol on HDL biochemical parameter in the long term.