Molecular Detection of the Genes gyrB, oprL, ETA, 16SrDNA in Pseudomonas Aeruginosa Strains Isolated from Clinical Samples of Karaj City Health Centers, Iran

Pseudomonas aeruginosa is one of the most important factors for nosocomial infections, particularly in immunosuppressed patients such as children. Study on the genes gyrB, oprL, ETA, 16SrDNA is essential to develop prevention programs; in this study, we tried to the study these genes in an Iranian population using multiplex-polymerase chain reaction (multiplex PCR) method. 55 different strains of Pseudomonas aeruginosa from clinical specimens collected from Karaj City Health Centers, Iran, after cultivation on the cetrimid agar and MacConkey agar media were detected via biochemical tests. DNA was extracted from bacterial genomics and the sequencing of target genes gyrB, oprL, ETA, 16SrDNA was amplified. Molecular test results showed that the frequencies of oprL, ETA, gyrB and 16SrDNA genes were 96.36, 94.50, 100 and 100 percent, respectively. The results show that the genes oprL and ETA are more sensitive and less specific for detecting Pseudomonas aeruginosa; but, using the genes gyrB and 16SrDNA has the most specificity. Simultaneous use of the genes oprL, ETA, 16SrDNA and gyrB would provide enough sensitivity to detect Pseudomonas aeruginosa from clinical specimens.