Neuroprotective effects of uric acid on axotomized spinal motoneurons in neonate rats
Abstract:
Introduction
Recently the neuroprotective role of uric acid (UA), as a natural biochemical substance, in neurodegenerative diseases has been proposed. Due to the critical role of apoptosis in many diseases of the nervous system, the present study was designed to investigate the anti-apoptotic effect of UA on motor neurons of the ventral spinal horn in neonate rats.Materials And Methods
Two-day-old rat neonates (n=10) were subdivided into experimental and control groups. In both groups, right sciatic nerve was transected. Experimental neonates were immediately treated with 10mg/kg UA intraperitoneally, while the controls wound was closed without treatment. Five days after axotomy, transverse sections of spinal cord at L5 level were prepared for morphometric cell count and TUNNEL assay.Results
The motor neuron loss in axotomized side compared to intact side was 30.48%, which illustrates the effectiveness of axotomy in inducing motoneuron cell death. The TUNNEL assay confirmed the apoptotic characteristics of cell death. Comparing the mean motoneurons loss in the axotomized side of the control and UA-treated neonates, revealed a significant difference between the two groups (pConclusion
Neuroprotective effect of UA is possibly through inhibiting the apoptotic cell death, which occurred with the axotomized motoneurons in ventral spinal horn.Keywords:
Language:
Persian
Published:
Koomesh, Volume:17 Issue: 3, 2016
Pages:
772 to 778
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