Optimizations of α-amylase production by response surface methodology in immobilization Bacillus amyloliquefaciens ATCC 23350

Production of an endogenous α-amylase from Bacillus amyloliquefaciens ATCC 23350 was studied and enhanced.
Protein and carbon sources were analyzed for free and immobilized bacterial cells and number of beads was considered for immobilized cells via one factor at a time methodforα-amylase production by Bacillus amyloliquefaciens. Subsequently, optimization condition was employed solely for immobilized bacterial cells by response surface methodology (RSM).
Peptone and rice starch showed to improve the α-amylase production in immobilized Bacillus cells. RSM generated a mathematical model explaining the optimum concentration of the efficient nutrients (139.35 g/l of rice starch and 80.00 g/l of peptone) leading to an optimum amylase production (205 U/ml).
Discussion and The statistical advance displayed significant outcomes to optimize the process parameters for maximal α-amylase production using Bacillus amyloliquefaciens and gave permission to rapid screening of variables. RSM led to find out an immense improvement in enzyme activity (more than 90%: from 25 to 225 U/ml) for the first time.