Identification of Resistant Chickpea Genotypes to Races 1,2,3,4 and 5 of Fusarium oxysporum f.sp ciceri, the Cause of Fusarium Wilt Using Molecular Markers

Fusarium wilt is considered as the second important pathogen of chickpea. Annual yield reduction caused by the disease has been estimated 10-90%. The ability of the pathogen to survive in soil for several years, even without the host, makes the control of disease very difficult. However, using chickpea cultivars that are resistant to fusarium wilt is the most effective and environment friendly method. In the present study, molecular markers (RAPD and SCAR) were used to identify chickpea genotypes containing the resistance genes to races of 1, 2, 3, 4 and 5. Genomic DNA was extracted using CTAB method from 42 chickpea genotypes. Then, polymerase chain reaction was carried out using CS-27 and CS-27700 molecular markers. Results showed that out of the 42 genotypes, 41 ones were susceptible to all the five tested races which had no resistance gene. Only Flip 06-152c line was resistant to these five races. The results of phenotypic resistance evaluation of genotypes under greenhouse condition confirmed the results of molecular evaluations.