Comparative study of sedation and pre-anesthetic effects of polar, semi polar and non-polar fractions of vinca minor extract in comparison with midazolam in rat

Periwinkle is a plant known as a Vinca minor in Persian. This plant contain bitter substance called Vincine, Tannin, Pectin, Carotene, vincamine Alkaloids, Vinci nine, Catarin, and a kind of Saponin and large amount of Vinine Alkaloids and Pubcine. All parts of this plant can be used; especially the leaves that can be picked in any season and seared by heat. The purpose of this study is evaluating sedation and pre-anesthetic effects of polar fractions, semi polar and non-polar of Vinca minor plant in comparison with midazolam found in rats. 60 Wistar rat head in different groups is applied in these tests. Three polar, semi-polar, and non-polar fractions and 0.04 mg/kg dose of midazolam and dimethyl sulfoxide (DMSO) with equal volume are transfused into peritoneum as a drug, half an hour before experiment. In a test the sedation and pre-anesthetic induction time and sleeping time effects are surveyed by seconds and recorded by chronometer. Findings show that in medical team the mentioned doses of extract meaningful both in infused dream by ketamine and increase in life time (p