Transfection Enhanced Green Florescent Protein to bovine spermatogonial colony through Turbofect

Spermatogonial Stem Cells (SSCs) are the only stem cells in adults that can transfer genetic information to future generations. Considering that a single SSC gives rise to a vast number of spermatozoa, genetic manipulation of these cells is a potential novel technology with feasible application to various animal species. The aim of this study was to evaluate Enhanced Green Fluorescent Protein (EGFP) gene transfection into bovine spermatogonial colonies via Turbofect carrier and assess the best incubation day in uptake exogenous gene by spermatogonial colonies. Transfection efficiency EGFP gene through Turbufect was determined different three days (day 4, 6 and 8) after the beginning of the culture by fluorescent microscope. Immunofluorescent staining against OCT4 and Vimentin led to the confirmation of the nature of both SSCs and sertoli cells. Results showed that the transfected colonies through Turbufect increased significantly (p