Evaluation of Freeze-Thawed Ghezel ram s spermatogonial cells colonization after co-culture with sertoli cells.

Spermatogenesis is a complex and coordinated process that is dependent upon the typeof stem cells known as spermatogonial stem cells. Few studies on culture of sheep spermatogonial stem cells are available. In this study, 3-5 tissue samples autopsied from testis of 2 month old male Ghezel rams after two stages of enzymatic digestion were divided into four groups: Group 1: ’Spermatogonial cells co-cultured with sertoli cells for 10 days, freeze-thawed in day 0’; group 2: ‘Spermatogonial cells co-cultured with sertoli cells for 10 days, freeze-thawed in day 14’; group 3: ‘Freeze-thawed spermatogonial cells in day 0 cultured for 10 days’; group 4: ’Freeze thawed spermatogonial cells in day 14 cultured for 10 days’ and control group: ‘Spermatogonial cells cocultured with day 0 fresh sertoli cells for 14 days’. The rate of colonization and colonies diameter were evaluated during these 10 days. Groups 3 and 4 did not show any signs of colonywhereas in group 1 a higher rate of colonization was observed comparing to group 2 (P