QTLs analysis controling physiological traits of barley under nickel stress

In order to map genomic regions controlling some physiological traits, an experiment was investigated in the Steptoe/Morex doubled haploid lines of barley with their parents under nickel stress and non stress conditions, Traits such as PI, Fv/Fm, Chlorophyll content, RWC, CMS, total Ni, Zn, Mn, Cu and Fe were measured. Qantitive Traits Loci analysis was carried out using genetic linkage map derived from 327 molecular marker of RFLP with composite interval mapping method. Analysis of variance showed significant difference between the lines of the studied traits in nickel stress and non stress conditions. The maximum correlation in non stress and stress conditions were observed between total Mn and Zn, respectively. We found 36 QTLs (20 and 16 QTLs for non stress and nickel stress conditions, respectively). For the studied traits. The minimum and maximum phenotypic variances which were explained by these QTLs changed from 10.09 (QMnk2H.1n) to 54.82 (QMnk7H.3n) percent, for total Mn in non stress condition. LOD scores were ranged from 2.53 to 4.55, for the Fv/Fm QTLs (QFv/Fm5H.1s and QFv/Fm5H.2s) and total Zn (QZnk6H.2s) in nickel stress condition respectively. P1 parent had higher role in transport of effective allele in physiological traits. QTLs QFek4H.n and QFek4H.1s is ranged from 48.50 to 49.50 cM of chromosome 4H, controlling total Fe, QTLs QNik5H.1n and QNik5H.1s in Location from 145.30 cM and QTLs QNik5H.2n and QNik5H.2s in place of 151.50 cM of chromosome 5H, controlling total Ni were quite stable. Therefore, if this result would repeat in different environment, years and genotypes, it can be used in marker assisted selection.