Effect of Testosteroneand PMSG Hormones on Organ Culture of Immature Testis of NMRI Mice

Spermatogenesis that including complicated and precise process of cell differentiation in mammals ,begins in early adulthood and continues throughout the life of reproduction , which resulted in dividing of fundamental cells , and result of dividing meiosis ,are haploid spermatids that basically have been chenged in their testicular and epididym because of creating high performance sperm.All of stages of spermatogenesis occur simultaneously, as in pathological conditions, the smallest disruption can lead to infertility.Beginning in terms of survival and spermatogenesis by FSH and testosterone hormones are regulated. Some researchers believe that the testosteronehormone is the main hormone for regulating of spermiogenesis and FSH has a minor role. PMSG has the similar performance to FSH. in this project we will consider the effects of sex hormones PMSG and testosterone on testicular tissue and spermatogenesis. IN VITRO experiments was done in three different groups including a controlt and two experimental groups on the immature male mice that were NMRI descent and duration of tissue culture was considered 4weeks.after passing specified time and doing histology and microscopy studying, the results indicated meaningful increase in number of spermatosist, spermatid, sperm and decrease in A spermatogonia Certoli cells. These results indicate that testicular tissue culture can be a useful experimental model for studying the regulatory mechanisms of proliferation of spermatogonia.