Association of two Common Single Nucleotide Polymorphisms (SNPs +45T/G and +276G/T) of ADIPOQ Gene with Coronary Artery Disease in Type 2 Diabetic Patients

Adiponectin, an adipocyte-secreted hormone, is known to have anti-atherogenic, anti-inflammatory, and anti-diabetic properties. In the present study, the association between two common single nucleotide polymorphisms (SNPs) ( and �蛽) of ADIOPQ gene and coronary artery disease (CAD) was assessed in the subjects with type 2 diabetes (T2DM).
Genotypes of two SNPs were determined by polymerase chain reaction-restriction fragment length polymorphism in 200 subjects with T2DM (100 subjects with CAD and 100 without CAD).
The frequency of TT genotype of �蛽 was significantly elevated in CAD compared to controls (χ2=7.967, P=0.019). A similar difference was found in the allele frequency of �蛽 between two groups (χ2=3.895, P=0.048). The increased risk of CAD was associated with � TT genotype when compared to reference GG genotype (OR=5.158; 95% CI=1.016-26.182, P=0.048). However, no similar difference was found in genotype and allele frequencies of SNP  between two groups. There was a CAD protective haplotype combination of � wild-type and mutant-type allele (276G-45G) (OR=0.37, 95% CI=0.16-0.86, P=0.022) in the subject population.
Our findings indicated that T allele of SNP �蛽 is more associated with the increased risk of CAD in subjects with T2DM. Also, a haplotype combination of 뿻滺 of these two SNPs has a protective effect on the risk of CAD.