Identification of Genomic Oregions Controlling Iron Concentration and Content in Shoot of Barley in A × B Doubled Hoploid Mapping Population

Iron is one of the essential micronutrient¡ which has an important role in nitrogen fixation and activity of some enzymes such as catalase¡ peroxidase and cytochrome oxidase. To map QTLs related to accumulation of iron in shoot of barley at five leaves and maturity stages¡ 148 doubled haploid lines derived from a cross between Clipper and Sahara3771 varieties were evaluated under greenhouse condition and single plant iron concentration and content were measured. For QTL analysis by linkage map including¡ 26 retrotransposone markers IRAP and REMAP¡ 246 SSR and EST-SSR¡ 238 RFLP and one morphological markers was used. Analysis of variance revealed significant difference between lines for all the studies traits and presence of trangreesive segregation for all the traits and indicated presence of desirable parental allele combinations in the progenies. In total¡ 511 markers in 7 linkage covered 1099.09 cM of barley genome with an average distance of 2.37 cM between two adjacent markers. For single plant iron concentration¡ eight and four¡ iron content in single plant¡ six and three QTLs were identified at vegetative and maturity stages¡ respectively. Negative additive effects of the most QTLs indicate the role of Sahara3771 alleles in increased iron accumulation in offspring. One common genomics regions was detected for QTLs of
single plant iron concentration and content at maturity which could be due to linkage between the QTLs or the pleiotropic effect of a single QTL.