Effect of Root Kudzu on Caspease-3 Activity by Immunohistochemy Method in the Testis in Streptozotocin-Induced Diabetic Rats

Apoptosis or programmed cell death plays an important role in the pathogenesis of many diseases. Diabetes mellitus is a devastating effect on reproductive system of male sexual function in human and animal models and increased apoptosis. Kudzu root is an ISO Flavonin and saponins and often is used as a reducing agent is glucose. Kudzu is able to lower blood glucose in non-insulin dependent and also, by eliminating free radicals that lead to the reduction of oxidative stress. This study was conducted on the effect of Kudzu roots on apoptosis in the testes of rats diabetic.
In this study, 32 male Wistar rats were randomly selected and divided into four groups: control, diabetic rats, diabetic rats treated with Kudzu 100 mg/kg, diabetic rats treated with Kudzu 50 mg / kg. Diabetic by intraperitoneal injection of streptozotocin55 mg/kg was induced. One week after injection, they were treated with a dose Kudzu 50 and 100 mg/kg for five weeks using gavage. Testicular damage by H & E staining and immunohistochemistry and hormonal and Blood biochemical factors were measured.
Findings: Diabetic rats showed a significant increase in apoptosis in the testis. Decrease in seminiferous tubule diameter of diabetic rats, Sertoli cells, sperm count,Insulin and testosterone levels were shown. In rats treated with Kudzu roots it resulted in significant reduction of apoptosis in diabetic rats and significantly increased body weight, plasma Insulin and testosterone levels. It was observed that the oral administration roots of Kudzu increase sperm count, sperm cell of Sertoli cells.
Discussion & The results of this study confirmed the occurrence of apoptosis in diabetes and root Kudzu of the anti-apoptotic effect notes.