Antimicrobial effect of chlorhexidine on aggregatibacterAggregatibacter actinomycetemcomitans biofilms associated with peri-implantitis

Background. This study was designedaimed to investigate assess the Antimicrobial antimicrobial eEffect of chlorhexidine (CHX) on Aggregatibacter A. actinomycetemcomitans biofilmss extracted isolated from subgingival plaque of Periperi-iImplantitis lesions.
Methods. 13 Thirteen Participants patients requiring peri-implantitis treatment were consecutively selected and theirwere consecutively recruited from patients referred for treatment of peri-implantitis. Patients’ subgingival biofilms were was obtained collected by inserting fine sterile paper points into peri-implant pocket and kept in place for 15 sseconds. A. actinomycetemcomitans was isolated from the This subgingival biofilm went under process and A. actinomycetemcomitans derived from special culturecultured. In this study, the standard strain of A. actinomycetemcomitans as served as the positive control group and a blank disk disc impregnated in with water as served as the negative control group were used.; Volume of 0.1 mLl of this the bacterial suspensions was incubated cultured on specific culture medium and. blank discs (6 mm diameter) impregnated with 0.2% CHX mouthrinse (Behsa Pharmaceutical Co.) impregnated blank disks (6 mm diameter) and negative control discs (not impregnated in 0.2% CHX) were placed on either the two sides of the bacterial culture plate. The size of zone of growth inhibition zone (the distance between the edge of the disc and the edge of the bacterial colony) was measured by a blinded, independent observer in millimetres.
Results. According to the results of disk disc diffusion test, the average mean diameter of growth inhibition zone of A. actinomycetemcomitans around disks discs impregnated with CHX showed significantwas larger zone of inhibition of A. actinomycetemcomitans growth in both standard bacterium(positive control) and biofilm samples of A. actinomycetemcomitans compared to the negative control group (blank diskdisc) (PConclusion. Using Use of 0.2% CHX mouthwash have has antibacterial effects on A. actinomycetemcomitans a species cultured isolated from peri-implantitis sites.