The study and identification of Quorum Sensing (QS) genes of Pseudomonas aeruginosa strains isolated from human clinical samples by Multiplex PCR and antibiotic resistance determination
Author(s):
Abstract:
Background
Pseudomonas aeruginosa is an opportunistic pathogen and the cause of 10% to 15% of ýnosocomial infections.Virulence genes of Pseudomonas aeruginosa is one of the most ýaggressive mechanisms and the issue of medical opinion is important. The expression of many ýgenes is controlled and regulated in pathogenic bacteria Pseudomonas aeruginosa gene by a ýsystem called Quorum Sensing (QS). QS controls and regulates cell-to-cell communication ýsystem using small molecules in single-celled organisms SMs. This study was aimed at ýdefining the prevalence of QS genes in Pseudomonas aeruginosa isolated from human ýresources.ýMaterials And Methods
Initially 60 samples of Pseudomonas aeruginosa from human intestinal samples were prepared ýand confirmed by culture-specific diagnostic tests. Multiplex PCR test was ýperformed to detect genes. Antimicrobial susceptibility of the isolates against 10 antimicrobial agents was ýdetermined using standard disk diffusion method.ýResults
Multiplex PCR results showed that the frequency of the desired genes rhlR 5 %, lasR 48.3% and lasI 60%. The genes lasB, apr, rhlAB and rhlI were not detected in any of the samples. According to antibiogram, the most resistance was against amikacin, amoxicillin and cefotaxime and the most susceptibility was to ciprofloxacin and ceftazidime.Conclusion
This study shows high prevalence of Quorom Sensing genes in Pseudomonas aeruginosa isolates of human origin.Keywords:
Language:
Persian
Published:
Yafteh, Volume:18 Issue: 2, 2016
Pages:
38 to 44
magiran.com/p1583134
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!