Plant regeneration of Citrus sinensis var. Thamson navel using sodium alginate-encapsulated shoot tips

Citrus genus is known as one of the world's major economic agricultural products . In the current research, a method is reported for a rtificial seed production technology by shoot tips in Citrus sinensis. var. Thamson navel . Alginate is one of the most efficient gelling agents in the production of capsule coating in artificial seeding technique. In the current research, impa ct of different constituents of a lginate matrix was investigated on a rtificial seed germination. In order to produce artificial seed s , shoot tips of Thamson navel were coated by three a lginate coating including SA 1 containing liquid MS and BA and NAA hormo nes, SA 2 with liquid MS without hormone , and SA3 with distilled water.Alginate coating with BA and NAA hormones was usedas control environment. The artificial seeds were then stored in the refrigerator (4 °C). Following passing storage period, seeds were placed in MS solid medium with BAP (10 mgl-1) and NAA (1 mgl-1) forconversion to seedlings. Using distilled water instead of MS medium for the preparation of a lginate coating significantly reduced the percentage of conversion in to seedlings.