The effect of hormone type and basic cultural media on multiplication and rooting of herbal species of Ribes khorasanicum

Ribes khorasanicum is a deciduous woody shrub form and is one of the most valuable species belongs to the monogenus family of Grossulariaceae. Ribes is a genus have about 150 species of flowering plants native throughout the temperate regions of the Northern Hemisphere. Because of the non-normative utilization, the utility of fruits, high palatability, livestock grazing, restricted ecological range, high ecological demands and sexual reproduction difficulties (mainly regenerates by vegetative method by rhizome) facing risk of extinction. One of the possible method of protection of the endangered taxon is multiplying and conservation of plants with help in vitro cultures. In this research effect of two sytokinin, BAP and Kinetin in presence or absence of auxin on proliferation coefficient and growth of multiplied shoots were investigated.Also, effect of nutrient concentration and sucrose concentration of cultural media, IBA and BAP hormones on rooting Percent and plantlet root length was investigated. Results showed that, BAP hormone has beter shoot multiplication index than kinetin, the most suitable culture media for multiplication was MS media supplemented by 1 mg/l BAP. For root induction best media was modified MS media which sucrose concentration reduced to 15 g/l, and supplemented with0.5 to 2 mg/l of IBA. Addition of 0.1 mg per liter BAP decreased the percentage of rooting and root length.The longest roots of plantlets was obtained from the modified MS media supplementedwith 2mg/l IBA. Plantlets were successfully transferred from tissue culture vessels to ex vitro conditionswith about 86 percent survival.