Synergistic Effects of Glomus mosseae and Sinorhizobium meliloti on Compatibility Metabolites of Alfalfa

Water deficit is one of the most important environmental stresses that adversely affect crop growth and production. Mycorrhizal fungi and symbiotic bacteria have important role in resistance to drought stress. A factorial experiment based on randomized complete block design was conducted with three replications in greenhouse, University of Mohaghegh Ardabil in 2013. Treatments were water stress at three levels ( 35%, 55% and 75% field capacity) and four seed inoculation as mycorrhiza (Glomus mosseae), Rhizobium (Sinorhizobium meliloti), mixed Inoculation (Glomus mosseae Sinorhizobium meliloti) and non-inoculated as control. The results showed that in severe stress, amount of proline, soluble sugars and water use efficiency increased (2.65 µmol/g FW, 2.21 mg/g FW, 1.1 g/kg, respectively) as compared to control. While the concentration of nitrogen, quantum yield, protein, osmotic potential and relative water content (0.57 %, 0.16, 0.8 mg/g.fwt, 27.81 % and - 0.6 bar) decreased, respectively. In between treatments including maximum and minimum amounts averages , soluble protein (5.17, 2.56 mg/g FW), soluble sugars (4.61, 3.55 mg/g FW) proline (5.8, 2.83 µmol/g FW), relative water content (81.61, 70.12(%, concentration of nitrogen (3.81, 2.66(% water use efficiency (0.86, 0.25 g/kg), respectively, were belonged to mixed inoculation and control. While the lowest amount of osmotic potential (-1.41 bar) related to mixed inoculation, and the most amount (-1.06 bar) to control. Quantum yield did not show significant difference in between the Inoculated treatments.