Gel-Based Proteomics

Proteomics has emerged since 1995 for studying the total proteome of cells, tissues, or organisms at any condition. The study of proteins has a fundamental importance because they are the functional and final molecules of the gene expression process and proteomics could be a valuable technique for achieving this purpose. Mass spectrometry is a complementary technique for proteomics that allows us to identify protein spots. In this review, we described all features of gel-based proteomics that include two different methods for separation and identification of protein spots.