The potential of catalase as an enzymatic antioxidant to improve freezability of Turkmen stallions sperm

Four mature stallions were used to study the effect of enzymatic antioxidant catalase on freezability of Turkmen stallion's sperm. Collected semen from stallions were processed and pooled before being divided treatments (200×106 sperm/ml) and diluted with extenders supplemented with different levels of catalase [0 (control), 100 U/ml (CAT100), 150U/ml (CAT150) and 200U/ml (CAT200)]. Extended and supplemented semen's were frozen according to a standard protocol. After thawing, motility, viability, membrane integrity, total abnormality and lipid peroxidation were assessed. Results showed that addition of 200U/ml catalase could improve progressive and total motility, viability, membrane integrity and decreased lipid peroxidation compared to control and other levels of catalase (p0.05). The results showed that different levels of catalase could not decreased total abnormality (p>0.05). We concluded that addition of 200U/ml catalase could improve freezability of Turkmen stallion’s sperm.