Exploring the Cytotoxicity Effects of Phlomis herba- venti Extract on HeLa Cancer Cell Line

In the last few decades, plants have been considered as one of the main sources of biologically active materials for the treatment of various diseases. Phlomis herba- venti belongs to Phlomis genus. There are different reports on various activities such as anti-inflammatory, immunosuppressive, anti-nociceptive, free radical scavenging, and antimicrobial effects of some of the species of Phlomis. This study aimed at investigating the in vitro cytotoxic activities of the crude ethanol extracts on HeLa cell line. The effect of ethanolic extract on the cell cytotoxic activity was evaluated using MTT assay. The cultivation media were affected by 0.156, 0.312, 0.625, 1.25, 2.5, 5, 7.5, and 10 mg/ml concentrations of crude extract. The optical density of the solution was quantified at 570 nm wavelengths by an ELISA Reader. In addition, the percentage of the growth inhibition of the cells exposed to extractions was calculated. The obtained results were analyzed by means of T-Test. The results indicated that the ethanolic extract with 2.5 and 10 mg/ml concentrations significantly decreased the proliferation of cancerous HeLa cells compared to the control group (p