Association Analysis for Morphological Traits in Cultivated Alfalfa using Molecular Markers
Author(s):
Abstract:
In the current investigation, 10 IRAP, 14 REMAP, 16 ISSR and 7 SSR primers and mixed linear model (MLM)-based association analysis were used to identify molecular markers associated with 13 morphological traits in cultivated alfalfa (Medicago sativa L.). Morphological traits studied, were height, chlorophyll content, total wet weight, total dry weight, wet leaf weight, dry leaf weight, wet stem weight, dry stem weight, number of leaves, number of stems, number of leaves/number of stems, dry leaf weight/dry stem weight and total dry weight/total wet weight. Analysis of genetic structure of the populations revealed 6 sub-populations (K=6). In general, MLM association analysis identified 95 markers associated with studied traits (P≤0.02). Fifty-four percentages of the associated markers were retrotransposon-based markers (IRAP and REMAP), while SSR and ISSR markers constituted 46 percentage of the associated markers. Marker ISSR14-4 was associated with 8 traits. The maximum number of markers (13) was associated with dry stem weight. Markers associated with dry stem weight and wet stem weight was also linked to the other studied traits.Results of the current study presented useful information about the genetic basis of the traits studied and can be used in alfalfa breeding programs to produce hybrids with high biomass.
Keywords:
Language:
Persian
Published:
Journal of Crop Breeding, Volume:8 Issue: 19, 2016
Pages:
52 to 60
magiran.com/p1636523
دانلود و مطالعه متن این مقاله با یکی از روشهای زیر امکان پذیر است:
اشتراک شخصی
با عضویت و پرداخت آنلاین حق اشتراک یکساله به مبلغ 1,390,000ريال میتوانید 70 عنوان مطلب دانلود کنید!
اشتراک سازمانی
به کتابخانه دانشگاه یا محل کار خود پیشنهاد کنید تا اشتراک سازمانی این پایگاه را برای دسترسی نامحدود همه کاربران به متن مطالب تهیه نمایند!
توجه!
- حق عضویت دریافتی صرف حمایت از نشریات عضو و نگهداری، تکمیل و توسعه مگیران میشود.
- پرداخت حق اشتراک و دانلود مقالات اجازه بازنشر آن در سایر رسانههای چاپی و دیجیتال را به کاربر نمیدهد.
In order to view content subscription is required
Personal subscription
Subscribe magiran.com for 70 € euros via PayPal and download 70 articles during a year.
Organization subscription
Please contact us to subscribe your university or library for unlimited access!