Study of Antibacterial Effect of Nano-Encapsulated Nisin and Natamycin in Liposoms against the growth of Staphylococcus aureus and Aspergillus niger

Nisin and natamycin have been used as antimicrobial agents in food and pharmaceutical applications. In its free form, nisin can react with reducing sugars and can non-specifically bind with lipids and proteins and hence its antibacterial activity is reduced. To overcome these limitations, using of liposomes has been reported. The main object of the present study was to study of antibacterial and antifungal Effect of nanoEncapsulated nisin and natamycin in liposoms against the growth of Staphylococcus aureus and Aspergillus niger. In this research, liposomes containing nisin and natamycin were produced. Then, particle size of nano-liposome, encapsulation efficiency and antimicrobial and antifungal effects of free and nano-encapsulated nisin and natamycin at tempreture of 8 and 25°C were investigated.
The results showed that particle size and size distribution (span) was 76-90 nm and 0.73-0.9 nm respectively. The encapsulation efficiency of nisin and natamaycin were 85-92% and 89-96%, respectively. The antimicrobial and antifungal results showed that use of encapsulated form of them, combination of bacteriocins and low tempreture storage is more effective. In this study nanoliposomes were prepared successfully by Mozaffari method. The Mozaffari method was effeicient method for encapsulation of nisin and natamyciny and evaluation of their antimicrobial and antifungal properties.