Considering dietary cation-anion changes on lactation and reproductive performances, milk fatty acids profile and mineral concentrations of serum in lactating dairy cows during heat stress

In order to survey the effect of three levels ofdietary cation-anion difference (DACD) on lactating performance, milk fatty acid profile, plasma and urine mineral concentration during heat stress. Eighteen Holstein cows (parity = 3, milk 43.5±1.5 Kg/d) were used in completely randomized design and randomly assigned into three treatments: (1) DACD (mEq/kg DM), (2) DACD (mEq/kg DM) and (3) DCAD (mEq/kg DM). Cows were fed experimental diets for 35 days starting from calving. Results showed dry matter intake (P