Effect of inoculation of Pseudomonas fluorescens FY32 bacteria to reduce the effects of salinity on canola (Brassica napus L.)

Salinity (sodium chloride) stress is a major environmental stress which along with drought stress is known to be the most effective abiotic stress which reducing crop production. Plant growth-promoting rhizobacteria (PGPR) colonize roots of monocots and dicots, and enhance plant growth by direct and indirect mechanisms. Modification of root system architecture by PGPR implicates the production of phytohormones and other signals that lead to enhanced plant growth. Inoculation of Plant Growth Promoting rhizobacteria and use of resistant varieties, are good practices to decline the negative effects of sodium chloride stress. This experiment was done to evaluate the effect of inoculation of Pseudomonas fluorescens FY32 in moderating the effects of salinity on canola varieties.
This experiment was conducted in hydroponic cultivation systems under split-split plot experimental design with three replications based on completely randomized design. Main factor is introduced as three levels of sodium chloride (0, 150, and 300 mM NaCl), Presence or absence of bacteria in the cultural system is sub factor and six varieties of canola is sub-sub factor (Hyola308, Sarigol, RGS003, Amica, Hyola420 and Olga respectively).
Statistical analysis indicated that growth indices (RGR, AGR, LAR,
NAR, LAD and LRGR) and leaf relative water content (RWC) were significantly
(P According to the analysis of the indices of growth and morpho-physiological data, it can therefore be concluded that the effect of sodium chloride stress reduced by inoculation of Pseudomonas fluorescens FY32 strain that produce ACC- deaminase enzyme. The presence of the bacteria and its symbiosis can promote plant growth in different ways and so the harmful effect of salinity and other abiotic stresses can be alleviated by the use of PGPR.