Grouping of some canola genotypes in various drought stress treatment in Germination Stages based on multivariate statistical methods

This study was conducted to grouping of 10 genotypes of canola via ISSR and IRAP markers and genotype resistance to drought. Eighty five polymorphic bands were produced using 19 individuals and combinations of ISSR and IRAP, and polymorphism varied between 50 to 100. Cluster analysis with UPGMA method placed the 10 genotypes in tree cluster, which includes 5, 3 and 2 respectively. Cluster analysis of germination results based on Euclidean distance with UPGMA method assigned treatment in four different groups consists of 11, 5, 9, and 15 genotypes respectively. Fisher’s linear Discriminant Function Analysis confirmed validity clustering analysis with 95%. The second group had the highest value, for most economical traits. SLM046 genotype could be introduced as resistant genotype by placing different treatments of its in one group. The fourth group included genotypes that had low value for most characteristics. Factor analysis results based on principal components showed that three factors named plumule, radicle and germination percentage, which determined 88.31 percent of total variation. First region was detected as good region on the base first and second factor evaluation. Genotypes in this area were favorable response to drought stress and could be introduced as resistant genotype at germination stage.