Evaluation of essential oil composition in three population of Hymenocrater calycinus( Boiss.) Benth. in different regions of Mazandaran province

Hymenocrater calycinus (Boiss.) Benth. is belongs to Lamiaceae family as an medicinal plant with antioxidant and antimicrobial properties, which has been wild distributed in rocky, Juniperus woodland and Artemisia steppe habitats in North, North East, East and Central of Iran (150-2500 m). In this research to purpose of evaluated of quality and quantity of plant essential oil, the aerial parts of plant in blooming were collected from three wild populations of Hymenocrater calycinus (Boiss.) Benth. (Baladeh paein, 1100 m; Baladeh bala, 1950 m and Orost, 1350 m) in Mazandaran province. Essential oils were obtained by hydro distillation method (Clevenger apparatus) and were analyzed by GC and GC/MS. A total of 56 compounds were identified in the essential oils of all H. calycinus populations. Most components of the plant were identified in the Baladeh bala site (37 compounds), while the highest yields was observed in the Orost site (1.27%). Hexadecanoic acid, spathulenol and 6, 10, 14-trimethyl-2-pentadecanone were the main components of essential oils in all three regions.