The molecular study of co- incidence of Avian Influenza (H9N2 subtype) and Metapneumovirus in Broiler Chickens with Respiratory Syndrome in Isfahan Province

At autumn of 2014 to 2015, lung and tracheal samples were taken from 35 broiler chickens flocks having respiratory syndrome suspected to avian influenza (H9N2 subtype) disease from different regions of Isfahan province. Viral RNA was extracted from tissue samples by RNA extraction kit and after synthesis of cDNA, avian influenza (H9N2 subtype) was detected with type and subtype specific primers. Therefore, a fragment approximately 115 base pair (bp) was amplified by one pair primers that been common to all avian pneumovirus (AP) viruses. Results showed 24 out of 35 (68.57%) of chicken flocks suffering to respiratory syndrome were infected to AI. The 11 out 24 flocks (45.83%) that infected to AI were co-infected to AP. The common morbidity of AI and AP in chicken flocks was 20.83%. Therefore it seems metapneumovirus has main role in forming and complexing of respiratory signs in broiler chickens suffering to AI in Isfahan province and complexing of AI and metapneumovirus infection may be possible in high percent of cases with respiratory syndrome.