Optimization of culture condition of enterobacter ZS extra cellular cyanide degrading enzyme with Response Surface Methodology

Although cyanide is a poisonous and lethal compound, it is used in several industries such as gold extraction. Industrial wastewater contains highly amounts of cyanide. That in order to degrade it, different chemical and physical methods are used. These methods not only include high expenses, but also if they combine with other materials, will produce more hazardous compound as well. On the other hand, utilizing biodegradation method, in addition to decreasing expenses, is environmentally friendly. In this study, bacterium was isolated from Muteh gold mining wastewater. Determination of morphological and biochemical characteristics and 16S rDNA described that the bacterium belong to Enterobacter genus. To achieve the maximum degradation by Enterobacter ZS, culture conditions has been optimized. The variables parameters that effect on the degradation procedure with maximum efficiency was achieved. By optimizing the values of affective factors and consider the interactions between factors, culture conditions were optimized by response surface methodology (RSM).