Immunohistochemical detection of apoptotic factors Bax and Bcl-2 in the lung alveoli following six weeks of high intensity exercise training

From the perspective of molecular studies¡ since the relationship between high intensity interval training and apoptosis-related proteins in the lungs has not investigated, aim of this study was immunohistochemical detection of apoptotic proteins Bax and Bcl-2 in the lungs alveoli after 6 weeks of high intensity interval training.
This research was an experimental study. Samples of this study were 14 male Wistar rats (4 weeks old¡ 72±8 gr weight), healthy and no history of disease that divided into training and control groups (exercise training=7¡ control=7). The high intensity interval training program was carried out for 6 weeks. Training program was started with 25 m/min and ended with 70 m/min speed at the final stage. In each training session, rats completed the 1-min activity with 10 repetition and work to rest ratio was 1:2. Lung tissue was extracted for Immunohistochemistry tests and protein levels of Bax and Bcl-2 were measured. To analyze data¡ independent t- test was used (P After 6 weeks of high intensity interval training¡ level of Bax protein significantly increased. Also, after 6 weeks of high intensity interval training¡ level of Bcl-2 protein also significantly increased (P It seems that intensity interval training period causes significant changes in expression of pulmonary alveolar apoptotic proteins and its accurate interpretation that it may be helpful or harmful require further researches.