The Effect of Pheromone (Z)-9-Tricosene On the Reproductive Organ in Male Wistar Rats

Biological stimulation and pheromone communication play an important role in reproduction process in the behavior of mammals. Pheromones can perceive via olfactory system in the urine, feces or from dermal skin glands, to stimulate both endocrine and behavioral reactions. The purpose of this study was to evaluate the effect of (Z)-9-Tricosene on the number of primary spermatocyte cells, spermatogonia, number of Leydig cells, epididymis tube diameter and thickness of seminiferous tube with different concentrations in male wistar rats.
In this study, four experimental groups were considered that consisted of three treatment groups and one control group. Treatment groups were in normal water condition and regular food and 1ml injection of three pheromone concentrations (100,200,300 micro gram per kilogram), respectively. And control group was exposed 1ml distilled water injected. Twenty eight injection was performed during eight weeks from above pheromone every other day, and some results were taken.
The results of our study indicated that after treatment with (Z)-9-tricosene pheromone, meaningful difference was observed between the number of primary spermatocyte and Leydig cells in the four study groups (P0.05).