Evaluation of E. coli On Testes Tissue study in Rat

There is more kind of reproductive infectious diseases. Viruses and bacterial agents can cause to presents this disease that occurs by sexual contact. Syphilis and Gonorrhea are the most common old known diseases that affects urogenital tract system, in nowadays HIV and transmitted Antigens by sexual contacts are the others reasons of urinary infectious diseases. The aim of current study was to investigate the effects of E. coli on testis tissue, seminiferous tubules and semen parameters. sixteen mature male Wistar rats with 200-220 grams’ weight and 2.5- 3 months’ ages, distributed to two of 8 rat’s groups and for 60 days of study period the lightning program was 12 hours’ light and 12 hours’ dark. Entro-toxigenic E coli (0114 serotype[n1] [PB2] ) was gained from Tehran Bouali reference laboratory for infect rats. After infection, samples were taken from testis tissue and Hematoxylin-eosin was used for histopathology. The motility, viability and semen count was evaluated. Histopathology studies indicated that in control group all seminiferous tubules were adhere together and all sexual germinal cells was seen. In E. coli infected group, seminiferous tubules were inconsecutive and germinal cells was not seen. Semen parameters evaluation showed that total count of sperm, viability and motility was decreased significantly in E. coli infected group in comparison to control group. The results indicated that the E. coli has undesirable effects on fertility rate and testis tissue and could decrease fertility rate.