Isolation of pathogenic mycoplasmas from Iranian ostrich farms

In ostriches, mycoplasmas are generally, related with respiratory diseases and causes rhino-tracheitis, airsacculitis and inflammation of the upper respiratory tract. The aim of current study was to isolation of pathogenic mycoplasmas in ostrich farms of Iran using PCR and culture methods. In this study, 100 samples were taken from lung, trachea and air sacs of ostriches with respiratory disease at slaughter time. Samples was evaluated by culture, rapid serum test and PCR. Results and The PCR tests results indicated 21.05% of samples was positive, which 7.89% and 14% was M. gallisepticum and M. synoviae, respectively. In culture method, 6.14% of samples was positive in view of M. gallisepticum and 7.01% of M. synoviae. Also according to clinical evaluation highest rate of positive cases was in lungs, air sacs, and trachea, respectively. According to prevalence of poultry mycoplasmas in ostriches, it is essential to exact evaluation of their pathogenesis in ostriches, to determine the importance of prevention.