Quantitative and qualitative extraction of stevioside from Stevia rebaudiana Bertoni. using solid phase molecularly imprinted technique

Stevia rebaudiana Bertoni. with non-calorie steviol glycoside is an effective drug to decrease of blood glucose in treat of diabetics. The goal of this study is obtaining of a novel method in order to a quantitative and qualitative extraction of stevioside as a non-calorie sweetener from Stevia. In this study the dry leaves of Stevia was prepared from Golsaran Shomal company and then in order to extraction of stevioside a solid phase molecularly imprinted extractor was prepared and some parameters affective on extraction of stevioside were optimized. An acrylate functional monomer and a cross linker were used to preparation of stevioside extractor polymer. Functional monomer to cross linker ratio and size and fining degree of the extractor were studied and evaluated. In this study analysis of the stevioside beside of HPLC was accrued by polarography method .The best ratio of functional monomer to cross linker was obtained in 0.2, also the best polymer particle size was obtained in 1000 micron for extraction of stevioside by the extractor and the amount of stevioside was determined 11.6% w/w in this plant.