Evaluating the Antimicrobial Properties of Pediococcus Stilesii Isolated From Whole Barley Sourdough
Author(s):
Abstract:
Background And Objectives
Non-aseptic fermentation ecosystems such as sourdough are the best substrate for lactic acid bacteria (LAB) with antimicrobial properties. This study was carried out to evaluate the antimicrobial properties of a dominant isolated LAB from whole barley sourdough against some food borne pathogens.Materials and Methods
In this experimental study after molecular identification of LAB isolate, the antimicrobial properties of the isolate and its cell free culture filtrate (CCF) as native and neutralized CCF, obtained from logarithmic and stationary phases were investigated. For this purpose, disc diffusion and microdilution (antibacterial) and also overlay and agar spore spot (antifungal) methods were used. Results were compared by the one way analysis of variance.Results
Sequencing results of PCR products lead to identification of Pediococcus stilesii as LAB isolate from barley sourdough. Results of investigating the antibacterial activities indicated that the highest antagonistic effect of the isolate and its native CCF obtained from the logarithmic phase were observed against Bacillus subtilis in comparison to other indicator bacteria (pConclusion
Based on the results of this study, it is possible to use from the P.stilesii and its CCF as bio preservative for controlling some microbial indicators in food and medical technologies.Keywords:
Language:
Persian
Published:
Iranian Journal of Nutrition Sciences & Food Technology, Volume:12 Issue: 3, 2017
Pages:
89 to 98
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