Evaluation of three Satureja species essential oil (S. macrantha, S. rechingeri, S. spicigera) against bacteria that cause hospital infections and Candida albicans

In order to evaluation of three Satureja species essential oils (S. macrantha, S. rechingeri, S. spicigera) against the bacteria that cause hospital infections and Candida albicans, in the first two accessions of every three species collected in 1391 from The Botanical Garden of Research Institute of Forests and Rangelands, the essential oil was isolated by water distillation . analyzed constituents of essential oils by GC/MS and the results show the essence of Satureja macrantha and Satureja spicigera, 16 components and essence of the Satureja rechingeri, 15 components were identified. The major compounds in the essential oils were Thymol, Carvacrol, p-cymene and -terpinene. In this experiment , essence dilution was in three levels including one-fifth, one twenty fifth and one fiftieth, and compare them with antibiotics (Ciprofloxacin and Ceftizoxime) and micro organisms at 5 levels , including Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans. The antibacterial activity was determined by disk diffusion method and estimate against five species. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the essential oils were determined with microdilution. Pseudomonas aeruginosa was the most resistant bacteria and Candida albicans Was more sensitive than bacteria. Satureja macrantha Showed the highest antimicrobial activity and Satureja spicigera showed the lowest antimicrobial activity.