The effects of different carbohydrate-whey protein ratio on IL-6 and IL-10 cytokines after high intensity exercise in healthy young girls

In previous studies, pro-inflammatory and anti-inflammatory cytokines were shown to increase after physical activity. The purpose of this study was to examine the effects of different carbohydrate-protein supplementation ratio on plasma levels of IL-6 and IL-10 after high-intensity exercise in healthy young females.
Twenty seven volunteer females aged 20-25 year divided into three groups: 1-placebo, 2- carbohydrate-protein (3:1), and carbohydrate-protein (4:1). After 12 h fasting, subjects have standard breakfast and then completed anaerobic test at 10 AM. Subjects received one of supplementations pre-, post-, and 2 h after test. Blood samples were taken before, immediately and 2 h after exercise. Data were analyzed by MANOVA and repeated measure ANOVA. Statistical significance was accepted at the 0.05 level.
IL-6 and IL-10 increased significantly after test and then 2 hours after test IL-6 decreased and IL-10 remained high in all groups. IL-10 and IL-6 cytokines didn’t differ between groups. Although, IL-10 was higher and IL-6 was lower in carbohydrate-protein (4:1) group than placebo group at two hours after exercise but those were not statistically significant.
carbohydrate-protein (4:1 ratio) supplementation resulted to some extent in improvements in cytokine responses at 2 hours after high-intensity exercise test.