Evaluation of the Proteome Profile Changes of Canola Leaf Inoculated With Pseudomonas Florescence FY32 under Salinity Stress

Plant growth-promoting bacteria enhance plant performance under stressful conditions using various mechanisms. This study was aimed to investigate the effects of Pseudomonas florescence FY32 on growth characteristics and to identify proteins involved in plant-bacterium interaction under salt stress. The results indicated that under salt stress (150 and 300 mM NaCl), plants inoculated with bacteria compared to non-inoculated plants possess better growth characteristics. Study of proteome pattern changes in leaf indicated that of 170 reproducible spots, 20 spots had differentially expression changes. Differentially expressed proteins were categorized into six functional groups, including energy metabolism (25%), antioxidants (20%), gene expression regulation (20%), photosynthesis (15%) and membrane proteins (1%). All of these proteins underwent same changes either in uninoculated or inoculated plants under salt stress, except Photosystem II CP47 (spot no. 1), Photosystem II CP43 (spot no. 2), and NAD(P)H-quinone oxidoreductase (spot no. 6) which had more expression level in inoculated plants than those non-inoculated plants. In general, results indicated that inoculation of Sarigol with the bacterium could improve its growth under salt stress.